Microscopy

MICRO MÉCANIQUE offers a comprehensive range of microscopy solutions, cameras, lenses, lighting, motorised stages, incubation chambers and accessories. We support you from selection to installation, including training and maintenance, to optimise your observations and protocols, from teaching laboratories to advanced research.

Electrophysiology & Neuroscience

For over 35 years, MICRO MÉCANIQUE SAS has been supporting researchers and laboratories in neuroscience, electrophysiology and optogenetics. We offer a comprehensive range of equipment (amplifiers, micromanipulators, microinjection systems, etc.) and comprehensive support: advice, installation, training and maintenance throughout France.

Camera ORCA Flash4.0 v3r Hamamatsu

The ORCA-Flash 4.0 v3 camera from Hamamatsu is designed for high-speed, high-sensitivity scientific imaging. It offers 4 MPixel resolution with 6.5 µm pixels and a large field of view. It can capture up to 100 frames/s at full resolution and up to 25,655 frames/s in lightsheet mode, thanks to ROI-optimised readout. Its quantum efficiency reaches 82% at 560 nm, with very high sensitivity between 400 and 900 nm. The camera has very low read noise (1 e- median at 40 fps) and a background noise of 0.06 e-/pixel/s. Digitisation is possible in 8, 12 or 16 bits. It offers different readout modes: normal, lightsheet and W-View, offering maximum flexibility in terms of regions of interest and exposure times.

ORCA Flash4.0 v3r Hamamatsu camera features

  • Resolution: 4 MPixels, 6.5 µm² pixels
  • Field of view: x3
  • Acquisition speed: 100 to 25,655 fps depending on ROI
  • ROI 2048 x 2048: 100 fps
  • ROI 2048 x 8: 25,655 fps
  • Quantum efficiency: 82% at 560 nm
  • Sensitivity: 400-900 nm
  • Median read noise: 1 e- @ 40 fps
  • Background noise: 0.06 e-/pixel/s
  • Digitisation: 8, 12 or 16 bits
  • Readout modes: normal, lightsheet, W-View
  • Independent readout for regions of interest and exposure directions

Applications

  • Time lapse with wide field of view
  • Very fast calcium imaging
  • Rtiometric imaging
  • Light sheet microscopy