Microscopy

MICRO MÉCANIQUE offers a comprehensive range of microscopy solutions, cameras, lenses, lighting, motorised stages, incubation chambers and accessories. We support you from selection to installation, including training and maintenance, to optimise your observations and protocols, from teaching laboratories to advanced research.

Electrophysiology & Neuroscience

For over 35 years, MICRO MÉCANIQUE SAS has been supporting researchers and laboratories in neuroscience, electrophysiology and optogenetics. We offer a comprehensive range of equipment (amplifiers, micromanipulators, microinjection systems, etc.) and comprehensive support: advice, installation, training and maintenance throughout France.

Monochrome Camera Orca FLASH 4.0 V3 Hamamatsu

Allows 100 images/s at full resolution and playback in light sheet mode (==> 25,655 images per second)

The Hamamatsu ORCA-Flash4.0 v3 is an ultra-fast, highly sensitive CMOS camera, ideal for advanced scientific imaging. It captures up to 100 fps in full resolution (2048 × 2048) and up to 25,655 fps in light sheet mode, while offering very low noise and broad spectral sensitivity (400-900 nm). With its large field of view, high quantum efficiency (82% at 560 nm) and various readout modes (normal, lightsheet, W-View), it excels for time-lapse, calcium imaging, ratiometry and lightsheet microscopy.

Features of the Orca FLASH 4.0 V3 Hamamatsu

  • CMOS sensor
  • Large field of view: x3
  • Cell size: 6.5 x 6.5 µm
  • Resolution: 2048 x 2048: 100 frames per second, 2048 x 8: 25,655 frames per second
  • Very high sensitivity between 400 and 900nm
  • Very low noise level (median read noise): 1e- 40 frames per second
  • Background noise: 0.06e/pixel/s
  • 8-bit, 12-bit, 16-bit digitisation
  • Playback options: normal mode, lightsheet mode, W-View mode (with independent exposure times between the 2 parts of the sensor, independent playback directions, independent regions of interest)
  • C-mount fitting
  • Quantum efficiency: 82% at 560 nm

Applications

  • Time lapse with wide field of view
  • Very fast calcium imaging
  • Rtiometric imaging
  • Light sheet microscopy

Documentation

Orca FLASH 4.0 V3 brochure

PDF file917.2 ko