Microscopy

MICRO MÉCANIQUE offers a comprehensive range of microscopy solutions, cameras, lenses, lighting, motorised stages, incubation chambers and accessories. We support you from selection to installation, including training and maintenance, to optimise your observations and protocols, from teaching laboratories to advanced research.

Electrophysiology & Neuroscience

For over 35 years, MICRO MÉCANIQUE SAS has been supporting researchers and laboratories in neuroscience, electrophysiology and optogenetics. We offer a comprehensive range of equipment (amplifiers, micromanipulators, microinjection systems, etc.) and comprehensive support: advice, installation, training and maintenance throughout France.

Camera Monochrome Orca Fusion CoaXPress 5.3M Time Lapse, Calcium and ratiometric imaging

The Hamamatsu ORCA-Fusion is a state-of-the-art scientific camera based on the 5.3-megapixel Gen III Scientific CMOS (sCMOS) sensor. Designed to combine high resolution, speed and extreme sensitivity, it boasts very low read noise (<1 e- rms), near-perfect linearity and an exceptional dynamic range (21,400:1). Thanks to its air/water cooling, multiple reading modes (normal, binning, lightsheet) and fast USB 3.0 and CoaXPress interfaces, it meets the most demanding needs in advanced microscopy.

Particularly well suited to low-light conditions, the ORCA-Fusion guarantees reliable detection of weak signals and images of exceptional quality. Its exclusive, patented “light sheet” mode considerably improves the signal-to-noise ratio, reducing photobleaching and phototoxicity.

Features of Orca Fusion CoaXPress 5.3M Time Lapse, Calcium and ratiometric imaging

  • Sensor: CMOS Gen III, 5.3 MP
  • Resolution: :

2304 × 2304 pixels @ 100 fps

2304 × 2048 pixels @ 100 fps

  • Pixel size: 6.5 × 6.5 µm
  • Read noise :

Fast scan: 1.4 e- rms

Standard scan: 1.0 e- rms

Slow scan: 0.7 e- rms

  • Background noise: 0.048 e- (RMS, silent scan)
  • Signal uniformity and quality :
  • Linearity: 99.67
  • PRNU: 0.06 % rms @ 7500 e-
  • DSNU: 0.3 e- rms
  • Quantum efficiency :

65 % @ 400 nm

80% @ 550 nm

70 % @ 700 nm

50 % @ 800 nm

  • Exposure time :

Fast scan: 17 µs – 10 s (step 4.87 µs)

Standard scan: 65 µs – 10 s (18.65 µs steps)

Slow scan: 280 µs – 10 s (step 80 µs)

  • Cooling: air & water (-5°C)
  • Dynamic range: 21,400:1
  • Playback modes: normal, binning (2×2, 4×4), lightsheet mode (patented)
  • Digitisation: 8, 12, 16 bits
  • Interface: CoaXPress (dual CXP-6) & USB 3.0
  • Data rate: 89 fps @ 5 MP

Applications

  • Advanced fluorescence microscopy
  • Long time-lapse imaging
  • Very fast calcium imaging
  • Ratiometric imaging
  • Light sheet microscopy
  • Studies of living cells and whole organisms

Documentation

Brochure

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