Microscopy

MICRO MÉCANIQUE offers a comprehensive range of microscopy solutions, cameras, lenses, lighting, motorised stages, incubation chambers and accessories. We support you from selection to installation, including training and maintenance, to optimise your observations and protocols, from teaching laboratories to advanced research.

Electrophysiology & Neuroscience

For over 35 years, MICRO MÉCANIQUE SAS has been supporting researchers and laboratories in neuroscience, electrophysiology and optogenetics. We offer a comprehensive range of equipment (amplifiers, micromanipulators, microinjection systems, etc.) and comprehensive support: advice, installation, training and maintenance throughout France.

MS-OPC Oocyte Recording Chamber ALA Scientific

The MS-OPC oocyte perfusion chamber is a device designed to efficiently channel perfusion solutions around an oocyte.

The oocyte is positioned at the bottom of a funnel, on a glass slide, where it is well bathed by the injection of solution via a metal cannula.

The funnel shape means that the volume at the bottom is minimal, guaranteeing very rapid exchange of solution while providing sufficient lateral space for the recording electrodes.

Features of the Oocyte Perfusion Chamber (MS-OPC)

  • Funnel shape to optimise oocyte contact with the solution, ensuring very rapid and homogeneous exchange
  • Positioning of the oocyte on the glass slide at the bottom of the cone for optimal bathing and electrical stability
  • 19-gage infusion cannula supplied, usable for perfusion or aspiration depending on assembly (with or without Levelock™ to manage overflow)
  • Reduced working volume for almost instantaneous solution change
  • Sufficient volume around the cone for placement of two voltage clamp recording electrodes

Technical characteristics of the Oocyte Perfusion Chamber (MS-OPC)

Caractéristique Valeur
Model MS-OPC
Type of room Funnel chamber oocyte infusion
Useful volume Very small (~4-20 µl), ensuring rapid infusion
Injection point 19-gage metal cannula (injection at the bottom of the cone)
Position of the oocyte On a glass slide (#1 cover slip) at the bottom of the cone
Fluid level management Funnel effect automatically controls infusion level
Compatibility Compatible with optical supports with side access for electrodes
Electrophysiological applications Use of patch clamp / two electrode voltage clamp on Xenopus oocytes
Cleaning / maintenance Simple assembly, easy removal for sterilisation and cleaning

Applications

  • Electrophysiological recordings on oocytes (Xenopus in particular) via voltage clamp or patch clamp
  • Pharmacological studies requiring rapid changes of solution around the oocyte
  • Laboratories and automated oocyte recording platforms
  • Research or teaching in the physiology and expression of ionic receptors

Documentation

ALA Scientific MS-OPC Manual

PDF file464 ko