Closed round chamber for MS-CPC imaging ALA Scientific
The MS-CPC closed perfusion chamber is specifically designed for perfusing cell cultures held on glass coverslips (#1 or #2 cover slip, ø 25 mm) during imaging experiments.
It forms a sealed enclosure thanks to a perforated O-ring between two coverslips, guaranteeing sterile isolation and the circulation of medium via integrated inlet and outlet ports.
Features of the ALA Scientific closed round chamber for MS-CPC imaging
- Closed design ensures tight control of the medium without contamination or evaporation
- Perforated elastomer O-ring (Buna-N or Viton) maintains two separate glass coverslips
- Easy evacuation via perforations in the O-ring or outlet tube
- Robust construction: stainless steel nest suitable for ø 35 mm Petri dish, stainless steel or Delrin top ring
Technical characteristics of the ALA Scientific round closed chamber for MS-CPC imaging
| Caractéristique | Valeur |
|---|---|
| Model | MS-CPC (Closed model with perforated O-ring) |
| Slat format | Round coverslips ø 25 mm, thickness #1 or #2 |
| Sealing gasket | Perforated square O-ring in Buna-N or Viton |
| Chamber volume | Very small (a few tens of µl) for rapid exchanges |
| Inlet pipe | PE-10 inserted via 19 GA needle through perforated O-ring |
| Fluid evacuation | By perforations in the O-ring or dedicated drainage tube |
| Materials | Nest in stainless steel, top ring in Delrin or stainless steel |
| Electrode accessibility | Low walls or side access |
| Compatibility | Petri dish ø 35 mm, MS-Stage stand, HCMIS micro-incubator |
| Use | Fluorescence or transmission imaging with controlled perfusion |
Applications
- Perfusion of cells or tissues on coverslips during imaging by optical or fluorescence microscopy
- Dynamic monitoring of viable cells under sterile conditions, without evaporation of the medium
- Experiments requiring rapid and stable solution exchange
- Compatible with temperature sensors or controlled environments (HCMIS, etc.)
Documentation
ALA Scientific MS-CPC brochure
PDF file – 105.4 ko