LED Fluorescent Illuminators pE-340fura – CoolLED
The pE-340fura LED illuminator is specially designed for ratiometric calcium imaging with the Fura-2 probe. Based on the proven platform of the pE-300 series, it combines performance, compactness and accessibility. With precise emissions at 340 nm and 380 nm, it provides high-quality calcium imaging, while also supporting conventional fluorescence (435-645 nm). Its design with no moving parts reduces electronic noise, improves the signal-to-noise ratio and reduces toxicity thanks to the use of lower concentrations of Fura-2.
Features of the pE-340fura
- Dedicated LED illumination for ratiometric imaging of Fura-2 calcium
- Main wavelengths: 340 nm & 380 nm
- Additional spectrum: 435-645 nm (GFP/FITC, TRITC/TxRed, mCherry, Cy5)
- Time resolution: microsecond
- Maximum reduction in electronic noise, no moving parts
- High signal-to-noise ratio ⇒ less Fura-2 required (reduced toxicity and costs)
- Maximum power with conventional microscopy configurations
- Single lamp alignment, no regular maintenance
- Direct connection to microscope or via liquid light guide
- Intensity adjustment: 0 to 100% (1% increments)
- Independent intensity control by colour
- Compatible with most imaging software
- TTL control with microsecond response time